Coding

Part:BBa_K2455000:Design

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-20)


His-tagged AroG gene from E. coli MG1655


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 241


Design Notes

In addition to having a P150L mutation the AroG sequence contains a his-tag in order to allow for detection of expression through the use of Western Blotting.

Source

The sequence originated from the MG1655 E. coli strain but carries a Pro150Leu point mutation in order to avoid negative feedback inhibition by L-phenylalanine (Gu et al., 2012), in addition a hexa his-tag has been added.

References

Gu, P. et al. (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. Microbial Cell Factories, 11(1), p.30.